If a researcher is interested in cloning only the protein coding parts of a gene, not the introns or promoter, what should the researcher do?
If a researcher is interested in cloning only the protein coding parts of a gene, not the introns or promoter, what should the researcher do?
A.Use DNA polymerase to transcribe the gene.
B.Use a restriction enzyme to cut the gene into shorter pieces.
C.Use reverse transcriptase to reconstruct a cDNA from the gene’s mRNA.
D.Use DNA ligase to join together only fragments of the DNA that code for the protein.
正确答案:Use reverse transcriptase to reconstruct a cDNA from the gene’s mRNA.
Tag:分子生物学
时间:2022-01-03 19:38:43
- 上一篇:A mutant of E. coli with a heat-sensitive DNA ligase (25℃ permissive, 37℃ nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant would demonstrate which of f
- 下一篇:Bacteria that contain ”empty” plasmid, (plasmid without the eukaryotic gene) would grow